Active Vitamin B12 (Holotranscobalamin) Test
- Category
- Vitamins
- Sample
- Blood sample taken from a vein in your arm
- Reports () Terms & Conditions Report time varies with time of sample collection.
- In 4 days
- Fasting
- No Fasting
- Age
- 5+ Years
What Is This Test?
Measures holotranscobalamin, the 'active' fraction of vitamin B12 that cells can actually use.
Why this test is performed?
Detects early or borderline B12 deficiency more reliably than total B12, especially when total B12 is in the grey zone.
Who should take this test?
Fatigue, weakness, tingling or numbness, mouth ulcers, hair fall, vegetarians/vegans, older adults and people with poor diet.
Test Preparation
No fasting needed.
Medical disclaimer · for information only, not medical advice
This article is for general information and education only. It is not medical advice, does not create a doctor–patient relationship, and is not a substitute for consultation with a qualified doctor who has assessed your individual situation. Never delay seeking medical advice because of something you have read here.
In a medical emergency, call 112, or 108 for an ambulance where that service operates, or go to your nearest emergency department. If you are in mental health distress, Tele-MANAS is free and available 24×7 on 14416.
Read our Editorial Policy and Medical Review Process.
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Frequently asked questions
When routine B12 is borderline or symptoms suggest deficiency despite a normal result.
No fasting is needed. You can eat and drink normally unless your doctor has advised otherwise.
Blood sample taken from a vein in your arm.
Usually within 2–3 days after the sample reaches the lab. You will receive the report online.
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