AFB Stain Test (Acid Fast Bacilli)

AFB Stain Test (Acid Fast Bacilli)

Category
Lungs
Sample
Sputum (deep-cough) sample, ideally first thing in the morning; other samples (fluid, pus) may be used
Reports () Terms & Conditions Report time varies with time of sample collection.
In 2 days
Fasting
No Fasting
Age
5+ Years

What Is This Test?

A sputum (or other) sample is stained and examined under the microscope for acid-fast bacilli (AFB) — the bacteria that cause tuberculosis.

Why this test is performed?

A rapid test for pulmonary TB in people with cough lasting more than 2 weeks, fever, weight loss or night sweats; also used to monitor TB treatment.

Who should take this test?

Cough for more than 2 weeks, breathlessness, wheezing, fever with chest symptoms, or TB exposure.

Test Preparation

No fasting needed.

Medical disclaimer · for information only, not medical advice

This article is for general information and education only. It is not medical advice, does not create a doctor–patient relationship, and is not a substitute for consultation with a qualified doctor who has assessed your individual situation. Never delay seeking medical advice because of something you have read here.

In a medical emergency, call 112, or 108 for an ambulance where that service operates, or go to your nearest emergency department. If you are in mental health distress, Tele-MANAS is free and available 24×7 on 14416.

Read our Editorial Policy and Medical Review Process.

Frequently asked questions

Rinse your mouth with water, take a deep breath and cough deeply from the chest — saliva alone is not suitable.

No fasting is needed. You can eat and drink normally unless your doctor has advised otherwise.

Sputum (deep-cough) sample, ideally first thing in the morning; other samples (fluid, pus) may be used.

Usually within 24–48 hours after the sample reaches the lab. You will receive the report online.

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